Conserved organization of centromeric chromatin in flies and humans

被引：426

作者：

Blower, MD

Sullivan, BA

Karpen, GH

机构：

[1] Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA

[2] Univ Calif San Diego, Dept Biol, La Jolla, CA 92037 USA

来源：

DEVELOPMENTAL CELL | 2002年 / 2卷 / 03期

关键词：

D O I：

10.1016/S1534-5807(02)00135-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent studies have highlighted the importance of centromere-specific histone H3-like (CENP-A) proteins in centromere function. We show that Drosophila CID and human CENP-A appear at metaphase as a three-dimensional structure that lacks histone H3. However, blocks of CID/CENP-A and H3 nucleosomes are linearly interspersed on extended chromatin fibers, and CID is close to H3 nucleosomes in polynucleosomal preparations. When CID is depleted by RNAi, it is replaced by H3, demonstrating flexibility of centromeric chromatin organization. Finally, contrary to models proposing that H3 and CID/CENP-A nucleosomes are replicated at different times in S phase, we show that interspersed H3 and CID/CENP-A chromatin are replicated concurrently during S phase in humans and flies. We propose that the unique structural arrangement of CID/CENP-A and H3 nucleosomes presents centromeric chromatin to the poleward face of the condensing mitotic chromosome.

引用

页码：319 / 330

页数：12

共 57 条

[31] A putative protein complex consisting of Ctf19, Mcm21 and Okp1 represents a missing link in the budding yeast kinetochore [J].