Measuring the viscoelastic properties of human platelets with the atomic force microscope

被引：677

作者：

Radmacher, M ^{[1
]}

Fritz, M ^{[1
]}

Kacher, CM ^{[1
]}

Cleveland, JP ^{[1
]}

Hansma, PK ^{[1
]}

机构：

[1] UNIV CALIF SANTA BARBARA, DEPT PHYS, SANTA BARBARA, CA 93106 USA

来源：

BIOPHYSICAL JOURNAL | 1996年 / 70卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0006-3495(96)79602-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have measured force curves as a function of the lateral position on top of human platelets with the atomic force microscope. These force curves show the indentation of the cell as the tip loads the sample. By analyzing these force curves we were able to determine the elastic modulus of the platelet with a lateral resolution of similar to 100 nm. The elastic moduli were in a range of 1-50 kPa measured in the frequency range of 1-50 Hz. Loading forces could be controlled with a resolution of 80 pN and indentations of the platelet could be determined with a resolution of 20 nm.

引用

页码：556 / 567

页数：12

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