Syk is required for integrin signaling in neutrophils

被引：363

作者：

Mócsai, A

Zhou, MJ

Meng, FY

Tybulewicz, VL

Lowell, CA ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA

[2] Natl Inst Med Res, London, England

来源：

IMMUNITY | 2002年 / 16卷 / 04期

关键词：

D O I：

10.1016/S1074-7613(02)00303-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The Syk tyrosine kinase plays a critical role in the signaling machinery of various receptors of the adaptive immune system. Here we show that Syk is also an essential component of integrin signaling in neutrophils. syk(-/-) neutrophils failed to undergo respiratory burst, degranulation, or spreading in response to proinflammatory stimuli while adherent to immobilized integrin ligands or when stimulated by direct crosslinking of integrins. Signaling from the beta(1), beta(2), or beta(3) integrins was defective in syk(-/-) cells. Syk colocalized with CD18 during cell spreading and initiated downstream signaling events leading to actin polymerization. Surprisingly, these defects in integrin-mediated activation did not impair the integrin-dependent in vitro or in vivo migration of syk(-/-) neutrophils or of cells deficient in Src-family kinases. Thus, integrins use different signaling mechanisms to support migration and adherent activation.

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页码：547 / 558

页数：12

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