Elaboration and use of nickel planar macrocyclic complex-based sensors for the direct electrochemical measurement of nitric oxide in biological media

被引：90

作者：

Bedioui, F ^{[1
]}

Trevin, S ^{[1
]}

Devynck, J ^{[1
]}

Lantoine, F ^{[1
]}

Brunet, A ^{[1
]}

Devynck, MA ^{[1
]}

机构：

[1] UNIV PARIS 05,URA 1482 CNRS,LAB PHARMACOL CARDIOVASC,F-75015 PARIS,FRANCE

来源：

BIOSENSORS & BIOELECTRONICS | 1997年 / 12卷 / 03期

关键词：

nitric oxide; blood platelets; endothelial cells; electrochemical detection; porphyrinic sensor; ultramicroelectrodes;

D O I：

10.1016/S0956-5663(97)85338-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We describe here the electrochemical detection of nitric oxide, NO, in biological systems by using chemically modified ultramicro carbon electrodes. In the first part of the paper, the different steps involved in the electrochemical preparation and characterization of the nickel-based sensor are described. This is illustrated by the use of nickel(II) tetrasulfonated phthalocyanine complex. The second part of the paper describes two examples of the direct electrochemical measurement of NO production in human blood platelets and endothelial cells from umbilical cord vein. (C) 1997 Elsevier Science Limited.

引用

页码：205 / 212

页数：8

共 39 条

[11] CULTURE OF HUMAN ENDOTHELIAL CELLS DERIVED FROM UMBILICAL VEINS - IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGICAL CRITERIA [J].

JAFFE, EA ;

NACHMAN, RL ;

BECKER, CG ;

MINICK, CR .

JOURNAL OF CLINICAL INVESTIGATION, 1973, 52 (11) :2745-2756

[12] SHEAR-STRESS INDUCES ATP-INDEPENDENT TRANSIENT NITRIC-OXIDE RELEASE FROM VASCULAR ENDOTHELIAL-CELLS, MEASURED DIRECTLY WITH A PORPHYRINIC MICROSENSOR [J].

KANAI, AJ ;

STRAUSS, HC ;

TRUSKEY, GA ;

CREWS, AL ;

GRUNFELD, S ;

MALINSKI, T .

CIRCULATION RESEARCH, 1995, 77 (02) :284-293

[13] DIRECT MEASUREMENT OF NITRIC-OXIDE PRODUCTION IN PLATELETS - RELATIONSHIP WITH CYTOSOLIC CA2+CONCENTRATION [J].

LANTOINE, F ;

BRUNET, A ;

BEDIOUI, F ;

DEVYNCK, J ;

DEVYNCK, MA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 215 (03) :842-848

[14] SELECTIVE AND SENSITIVE ELECTROCHEMICAL MEASUREMENT OF NITRIC-OXIDE IN AQUEOUS-SOLUTION - DISCUSSION AND NEW RESULTS [J].

LANTOINE, F ;

TREVIN, S ;

BEDIOUI, F ;

DEVYNCK, J .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1995, 392 (1-2) :85-89

[15]

Leone A M, 1995, Methods Mol Biol, V41, P285

[16] Detection of endothelial cell-derived nitric oxide: Current trends and future directions [J].

Magazine, HI .

ADVANCES IN NEUROIMMUNOLOGY, 1995, 5 (04) :479-490

[17] MEASUREMENTS OF NITRIC-OXIDE IN BIOLOGICAL-MATERIALS USING A PORPHYRINIC MICROSENSOR [J].

MALINSKI, T ;

TAHA, Z ;

GRUNFELD, S ;

BUREWICZ, A ;

TOMBOULIAN, P ;

KIECHLE, F .

ANALYTICA CHIMICA ACTA, 1993, 279 (01) :135-140

[18] NITRIC-OXIDE SYNTHASE ACTIVITY IN GENETIC-HYPERTENSION [J].

MALINSKI, T ;

KAPTURCZAK, M ;

DAYHARSH, J ;

BOHR, D .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 194 (02) :654-658

[19] NITRIC-OXIDE MEASURED BY A PORPHYRINIC MICROSENSOR IN RAT-BRAIN AFTER TRANSIENT MIDDLE CEREBRAL-ARTERY OCCLUSION [J].

MALINSKI, T ;

BAILEY, F ;

ZHANG, ZG ;

CHOPP, M .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1993, 13 (03) :355-358

[20] DIRECT ELECTROCHEMICAL MEASUREMENT OF NITRIC-OXIDE RELEASED FROM HUMAN PLATELETS [J].

MALINSKI, T ;

RADOMSKI, MW ;

TAHA, Z ;

MONCADA, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 194 (02) :960-965

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