A review of fluorescence methods for assessing labile iron in cells and biological fluids

被引：218

作者：

Espósito, BP ^{[1
]}

Epsztejn, S ^{[1
]}

Breuer, W ^{[1
]}

Cabantchik, ZI ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Life Sci, Dept Biol Chem, IL-91904 Jerusalem, Israel

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 304卷 / 01期

关键词：

iron; fluorescence; oxidative stress; transport; transferrin; chelators;

D O I：

10.1006/abio.2002.5611

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A variety of biochemical, pharmacological, and toxicological properties have been attributed to labile forms of iron that are associated with cells or with biological fluids. Unlike the major fraction of bioiron which is protein bound, the labile bioiron is chelatable and therefore amenable for detection by metal-sensing devices that are coupled to chelation moieties. The present review deals with the detection of various labile forms of iron present in living cells and in fluids of biological interest, in health and disease. The main focus of the review is on the design and application of fluorescent probes as analytical tools for assessing labile iron and iron transport mechanisms and the efficiency of iron chelators in solution and in cellular systems. (C) 2002 Elsevier Science (USA).

引用

页码：1 / 18

页数：18

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