Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals

被引:216
作者
Cowell, IG
Aucott, R
Mahadevaiah, SK
Burgoyne, PS
Huskisson, N
Bongiorni, S
Prantera, G
Fanti, L
Pimpinelli, S
Wu, R
Gilbert, DM
Shi, W
Fundele, R
Morrison, H
Jeppesen, P
Singh, PB [1 ]
机构
[1] Roslin Inst, Nucl Reprogramming Lab, Div Gene Express & Dev, Roslin EH25 9PS, Midlothian, Scotland
[2] Natl Inst Med Res, Lab Dev Genet, London NW7 1AA, England
[3] Severn Biotech, Kidderminster DY11 6JT, Worcs, England
[4] Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy
[5] Univ Roma La Sapienza, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy
[6] SUNY Upstate Med Univ, Dept Biochem & Mol Biol, Syracuse, NY 13210 USA
[7] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
[8] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1007/s00412-002-0182-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (X-i) in female cells, as well as in the XY body during meiosis in the male, and forms a G-band pattern along the arms of the autosomes. Me9H3 is a constituent of imprinted chromosomes that are repressed. The paternal and maternal pronuclei in one-cell mouse embryos show a striking non-equivalence in Me9H3: the paternal pronucleus contains no immunocytologically detectable Me9H3. The levels of Me9H3 on the parental chromosomes only become equivalent after the two-cell stage. Finally, we provide evidence that Me9H3 is neither necessary nor sufficient for localisation of heterochromatin protein 1 (HP1) to chromosomal DNA.
引用
收藏
页码:22 / 36
页数:15
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