The protein CTCF is required for the enhancer blocking activity of vertebrate insulators

被引：877

作者：

Bell, AC ^{[1
]}

West, AG ^{[1
]}

Felsenfeld, G ^{[1
]}

机构：

[1] NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

CELL | 1999年 / 98卷 / 03期

关键词：

D O I：

10.1016/S0092-8674(00)81967-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An insulator is a DNA sequence that can act as a barrier to the influences of neighboring cis-acting elements, preventing gene activation, for example, when located between an enhancer and a promoter. We have identified a 42 bp fragment of the chicken beta-globin insulator that is both necessary and sufficient for enhancer blocking activity in human cells. We show that this sequence is the binding site for CTCF, a previously identified eleven-zinc finger DNA-binding protein that is highly conserved in vertebrates. CTCF sites are present in all of the vertebrate enhancer-blocking elements we have examined. We suggest that directional enhancer blocking by CTCF is a conserved component of gene regulation in vertebrates.

引用

收藏

页码：387 / 396

页数：10

相关论文

共 38 条

[21] A POSITION-EFFECT ASSAY FOR BOUNDARIES OF HIGHER-ORDER CHROMOSOMAL DOMAINS [J].

KELLUM, R ;

SCHEDL, P .

CELL, 1991, 64 (05) :941-950

[22] A GROUP OF SCS ELEMENTS FUNCTION AS DOMAIN BOUNDARIES IN AN ENHANCER-BLOCKING ASSAY [J].

KELLUM, R ;

SCHEDL, P .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (05) :2424-2431

[23] Molecular weight abnormalities of the CTCF transcription factor: CTCF migrates aberrantly in SDS-PAGE and the size of the expressed protein is affected by the UTRs and sequences within the coding region of the CTCF gene [J].

Klenova, EM ;

Nicolas, RH ;

U, S ;

Carne, AF ;

Lee, RE ;

Lobanenkov, VV ;

Goodwin, GH .

NUCLEIC ACIDS RESEARCH, 1997, 25 (03) :466-473

[24] CTCF, A CONSERVED NUCLEAR FACTOR REQUIRED FOR OPTIMAL TRANSCRIPTIONAL ACTIVITY OF THE CHICKEN C-MYC GENE, IS AN 11-ZN-FINGER PROTEIN DIFFERENTIALLY EXPRESSED IN MULTIPLE FORMS [J].

KLENOVA, EM ;

NICOLAS, RH ;

PATERSON, HF ;

CARNE, AF ;

HEATH, CM ;

GOODWIN, GH ;

NEIMAN, PE ;

LOBANENKOV, VV .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (12) :7612-7624

[25] DNA-SEQUENCES FOR TYPICAL RIBOSOMAL GENE SPACERS FROM XENOPUS-LAEVIS AND XENOPUS-BOREALIS [J].

LABHART, P ;

REEDER, RH .

NUCLEIC ACIDS RESEARCH, 1987, 15 (08) :3623-3624

[26]

LOBANENKOV VV, 1990, ONCOGENE, V5, P1743

[27] GAGA mediates the enhancer blocking activity of the eve promoter in the Drosophila embryo [J].

Ohtsuki, S ;

Levine, M .

GENES & DEVELOPMENT, 1998, 12 (21) :3325-3330

[28] Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators [J].

Pikaart, MI ;

Recillas-Targa, F ;

Felsenfeld, G .

GENES & DEVELOPMENT, 1998, 12 (18) :2852-2862

[29] An insulator element and condensed chromatin region separate the chicken β-globin locus from an independently regulated erythroid-specific folate receptor gene [J].

Prioleau, MN ;

Nony, P ;

Simpson, M ;

Felsenfeld, G .

EMBO JOURNAL, 1999, 18 (14) :4035-4048

[30] DEVELOPMENTAL REGULATION OF TOPOISOMERASE-II SITES AND DNASE I-HYPERSENSITIVE SITES IN THE CHICKEN-BETA-GLOBIN LOCUS [J].

REITMAN, M ;

FELSENFELD, G .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (06) :2774-2786

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