Dicer is essential for mouse development

被引：1478

作者：

Bernstein, E

Kim, SY

Carmell, MA

Murchison, EP

Alcorn, H

Li, MZ

Mills, AA

Elledge, SJ

Anderson, KV

Hannon, GJ

机构：

[1] Watson Sch Biol Sci, Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[2] SUNY Stony Brook, Genet Program, Stony Brook, NY 11794 USA

[3] Sloan Kettering Inst, Dev Biol Program, New York, NY 10021 USA

[4] Baylor Coll Med, Dept Biochem & Mol Biol, Houston, TX 77030 USA

[5] Baylor Coll Med, Howard Hughes Med Inst, Houston, TX 77030 USA

来源：

NATURE GENETICS | 2003年 / 35卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/ng1253

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To address the biological function of RNA interference (RNAi) related pathways in mammals, we disrupted the gene Dicer1 in mice. Loss of Dicer1 lead to lethality early in development, with Dicer1 - null embryos depleted of stem cells. Coupled with our inability to generate viable Dicer1 - null embryonic stem (ES) cells, this suggests a role for Dicer, and, by implication, the RNAi machinery, in maintaining the stem cell population during early mouse development.

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收藏

页码：215 / 217

页数：3

相关论文

共 15 条

[1] Multipotent cell lineages in early mouse development depend on SOX2 function [J].

Avilion, AA ;

Nicolis, SK ;

Pevny, LH ;

Perez, L ;

Vivian, N ;

Lovell-Badge, R .

GENES & DEVELOPMENT, 2003, 17 (01) :126-140

[2] Role for a bidentate ribonuclease in the initiation step of RNA interference [J].

Bernstein, E ;

Caudy, AA ;

Hammond, SM ;

Hannon, GJ .

NATURE, 2001, 409 (6818) :363-366

[3] Crystallographic and modeling studies of RNase III suggest a mechanism for double-stranded RNA cleavage [J].

Blaszczyk, J ;

Tropea, JE ;

Bubunenko, M ;

Routzahn, KM ;

Waugh, DS ;

Court, DL ;

Ji, XH .

STRUCTURE, 2001, 9 (12) :1225-1236

[4] The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis [J].

Carmell, MA ;

Xuan, ZY ;

Zhang, MQ ;

Hannon, GJ .

GENES & DEVELOPMENT, 2002, 16 (21) :2733-2742

[5] Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C-elegans developmental timing [J].

Grishok, A ;

Pasquinelli, AE ;

Conte, D ;

Li, N ;

Parrish, S ;

Ha, I ;

Baillie, DL ;

Fire, A ;

Ruvkun, G ;

Mello, CC .

CELL, 2001, 106 (01) :23-34

[6] RNA interference [J].

Hannon, GJ .

NATURE, 2002, 418 (6894) :244-251

[7] A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA [J].

Hutvágner, G ;

McLachlan, J ;

Pasquinelli, AE ;

Bálint, É ;

Tuschl, T ;

Zamore, PD .

SCIENCE, 2001, 293 (5531) :834-838

[8]

HUTVAGNER G, 2002, SCIENCE, V1, P1

[9] Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C-elegans [J].

Ketting, RF ;

Fischer, SEJ ;

Bernstein, E ;

Sijen, T ;

Hannon, GJ ;

Plasterk, RHA .

GENES & DEVELOPMENT, 2001, 15 (20) :2654-2659

[10] A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans [J].

Knight, SW ;

Bass, BL .

SCIENCE, 2001, 293 (5538) :2269-2271