Effect of Hypoxic Preconditioning on Neural Cell Apoptosis and Expression of Bcl-2 and Bax in Cerebral Ischemia-Reperfusion in Rats

被引：6

作者：

高晓群 ^{[1
]}

常成 ^{[1
]}

段东晓 ^{[1
]}

茹立强 ^{[2
]}

殷光甫 ^{[2
]}

机构：

[1] Department of Anatomy, Medical College of Zhengzhou University, Zhengzhou , China

[2] Institution of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan , China

来源：

华中科技大学学报(医学英德文版) | 2006年 / 01期

关键词：

hypoxic preconditioning; cerebral ischemia; apoptosis; Bcl-2; Bax;

D O I：

暂无

中图分类号：

R743.3 [急性脑血管疾病（中风）];

学科分类号：

1002 ;

摘要：

In order to investigate the protective effect of hypoxic preconditioning on the cerebral ischemia-reperfusion injury, the expression of Bcl-2 and Bax was detected by using immunohistochemical staining after 3 h cerebral ischemia followed by 1, 6, 12, 24 and 48 h reperfusion respectively in rats treated with or without hypoxic preconditioning before cerebral ischemia. In addition, the apoptosis of neural cells and the behavioral scores for neurological functions recovery were evaluated by TUNEL staining and “crawling method”, respectively. Compared with control group (cerebral ischemia-reperfusion without hypoxic preconditioning), the expression of Bcl-2 was significantly increased, but that of Bax decreased in the hypoxic preconditioning group (cerebral ischemia-reperfusion with hypoxic preconditioning), both P<0.05. The pre-treatment with hypoxic preconditioning could reduce the apoptosis of neural cells and promote the neurological function recovery as compared to control group. It was suggested that hypoxic preconditioning may have protective effects on the cerebral ischemia-reperfusion injury by inhibiting the apoptosis of neural cells, increase the expression of Bcl-2 and decrease the expression of Bax.

引用

页码：17 / 20

页数：4