高钾可诱导培养的小脑颗粒神经元凋亡

被引：5

作者：

李文明

苏兴文

邱鹏新

颜光美

机构：

[1] 中山医科大学药理学教研室!广东广州,中山医科大学药理学教研室!广东广州,中山医科大学药理学教研室!广东广州,中山医科大学药理学教研室!广东广州

来源：

中国神经科学杂志 | 2001年 / 01期

基金：

广东省自然科学基金;

关键词：

高钾; 谷氨酸; 神经元; 凋亡;

D O I：

暂无

中图分类号：

R338.1 [神经原生理学];

学科分类号：

0710 ; 071006 ;

摘要：

采用四唑盐比色、琼脂糖凝胶电泳、乙二酸荧光素染色和Hoechst332 58染色等方法研究高钾对原代培养的大鼠小脑颗粒神经元的毒性作用及其机制。结果发现 :①高钾诱导神经元死亡呈剂量 ( 50～ 10 0mmol/L)和时间依赖性 ;②神经元死亡呈现明显的凋亡特征 :胞体缩小 ,染色质浓缩 ,DNA“梯形”条带形成和蛋白质合成抑制剂 (cycloheximide ,1.0mg/L)可阻断其毒性等 ;③MK 80 1( 2 μmol/L)、尼莫地平 ( 10 μmol/L)、硫酸镁 ( 2 0mmol/L)可阻断高钾的大部分毒性作用。结果提示 :高钾可能通过刺激内源性谷氨酸释放从而诱导小脑颗粒神经元凋亡

引用

页码：88 / 92+100 +100

页数：6

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