EVIDENCE THAT CYTOCHROME-P450 2E1 IS INVOLVED IN THE (OMEGA-1)-HYDROXYLATION OF LAURIC ACID IN RAT-LIVER MICROSOMES

被引：71

作者：

AMET, Y ^{[1
]}

BERTHOU, F ^{[1
]}

GOASDUFF, T ^{[1
]}

SALAUN, JP ^{[1
]}

LEBRETON, L ^{[1
]}

MENEZ, JF ^{[1
]}

机构：

[1] UNIV STRASBOURG 1,INST BIOL MOLEC & CELLULAIRE PLANTES,CNRS,UA 1182,STRASBOURG,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.2305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study examined changes in hepatic CYP2E1 content and (omega-1)-hydroxylation of lauric acid in rats treated with pyridine, pyrazole, acetone, ethanol and 3-methylcholanthrene. The (omega-1)-hydroxylase activity was strongly correlated with chlorzoxazone 6-hydroxylation (r = 0.76) and 4-nitrophenol-hydroxylase (r = 0.31). Both these activities are carried out by CYP2E1. (omega-1) hydroxylase activity was inhibited by ethanol (Ki = 3.5 mM), dimethylsulfoxide and diethyldithiocarbamate. Furthermore, polyclonal antibody directed against rat CYP2E1 inhibited (omega-1)-hydroxylation by more than 90 % while it had no effect on the omega-hydroxylation. These results suggest that the (omega-1)-hydroxylation of lauric acid is mediated principally by the CYP2E1 enzyme in rat liver microsomes. (C) 1994 Academic Press, Inc.

引用

页码：1168 / 1174

页数：7

共 20 条

[11]

MA XL, 1993, HEPATOLOGY, V18, P1247

[12] EXPRESSION OF CYTOCHROME-P-450 ENZYMES IN CULTURED HUMAN HEPATOCYTES [J].

MOREL, F ;

BEAUNE, PH ;

RATANASAVANH, D ;

FLINOIS, JP ;

YANG, CS ;

GUENGERICH, FP ;

GUILLOUZO, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 191 (02) :437-444

[13] THE P450 SUPERFAMILY - UPDATE ON NEW SEQUENCES, GENE-MAPPING, ACCESSION NUMBERS, EARLY TRIVIAL NAMES OF ENZYMES, AND NOMENCLATURE [J].

NELSON, DR ;

KAMATAKI, T ;

WAXMAN, DJ ;

GUENGERICH, FP ;

ESTABROOK, RW ;

FEYEREISEN, R ;

GONZALEZ, FJ ;

COON, MJ ;

GUNSALUS, IC ;

GOTOH, O ;

OKUDA, K ;

NEBERT, DW .

DNA AND CELL BIOLOGY, 1993, 12 (01) :1-51

[14]

OKITA RT, 1980, DRUG METAB DISPOS, V8, P147

[15] EFFECTS OF ETHANOL AND CLOFIBRATE ON EXPRESSION OF CYTOCHROME-P-450 ENZYMES AND EPOXIDE HYDROLASE IN CULTURES AND COCULTURES OF RAT HEPATOCYTES [J].

PERROT, N ;

CHESNE, C ;

DEWAZIERS, I ;

CONNER, J ;

BEAUNE, PH ;

GUILLOUZO, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 200 (01) :255-261

[16] HYDROXYLATION OF CHLORZOXAZONE AS A SPECIFIC PROBE FOR HUMAN LIVER CYTOCHROME-P-450IIE1 [J].

PETER, R ;

BOCKER, R ;

BEAUNE, PH ;

IWASAKI, M ;

GUENGERICH, FP ;

YANG, CS .

CHEMICAL RESEARCH IN TOXICOLOGY, 1990, 3 (06) :566-573

[17] DIFFERENTIAL INACTIVATION OF PLANT LAURIC ACID RHO-CHAIN AND IN-CHAIN-HYDROXYLASES BY TERMINALLY UNSATURATED FATTY-ACIDS [J].

SALAUN, JP ;

SIMON, A ;

DURST, F ;

REICH, NO ;

DEMONTELLANO, PRO .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 260 (02) :540-545

[18] MULTIPLE FORMS OF HEPATIC CYTOCHROME-P-450 - PURIFICATION, CHARACTERIZATION AND COMPARISON OF A NOVEL CLOFIBRATE-INDUCED ISOZYME WITH OTHER MAJOR FORMS OF CYTOCHROME-P-450 [J].

TAMBURINI, PP ;

MASSON, HA ;

BAINS, SK ;

MAKOWSKI, RJ ;

MORRIS, B ;

GIBSON, GG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 139 (02) :235-246

[19] OMEGA-HYDROXYLATION AND (OMEGA-1)-HYDROXYLATION OF ARACHIDONIC-ACID, LAURIC ACID AND PROSTAGLANDIN-A1 BY MULTIPLE FORMS OF CYTOCHROME-P-450 PURIFIED FROM RAT HEPATIC MICROSOMES [J].

TANAKA, S ;

IMAOKA, S ;

KUSUNOSE, E ;

KUSUNOSE, M ;

MAEKAWA, M ;

FUNAE, Y .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1043 (02) :177-181

[20]

TASSANEEYAKUL V, 1993, BIOCHEM PHARMACOL, V46, P1975

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