EVIDENCE THAT CYTOCHROME-P450 2E1 IS INVOLVED IN THE (OMEGA-1)-HYDROXYLATION OF LAURIC ACID IN RAT-LIVER MICROSOMES

被引：71

作者：

AMET, Y ^{[1
]}

BERTHOU, F ^{[1
]}

GOASDUFF, T ^{[1
]}

SALAUN, JP ^{[1
]}

LEBRETON, L ^{[1
]}

MENEZ, JF ^{[1
]}

机构：

[1] UNIV STRASBOURG 1,INST BIOL MOLEC & CELLULAIRE PLANTES,CNRS,UA 1182,STRASBOURG,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.2305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study examined changes in hepatic CYP2E1 content and (omega-1)-hydroxylation of lauric acid in rats treated with pyridine, pyrazole, acetone, ethanol and 3-methylcholanthrene. The (omega-1)-hydroxylase activity was strongly correlated with chlorzoxazone 6-hydroxylation (r = 0.76) and 4-nitrophenol-hydroxylase (r = 0.31). Both these activities are carried out by CYP2E1. (omega-1) hydroxylase activity was inhibited by ethanol (Ki = 3.5 mM), dimethylsulfoxide and diethyldithiocarbamate. Furthermore, polyclonal antibody directed against rat CYP2E1 inhibited (omega-1)-hydroxylation by more than 90 % while it had no effect on the omega-hydroxylation. These results suggest that the (omega-1)-hydroxylation of lauric acid is mediated principally by the CYP2E1 enzyme in rat liver microsomes. (C) 1994 Academic Press, Inc.

引用

页码：1168 / 1174

页数：7

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