PURIFICATION AND CHARACTERIZATION OF A BENZENE HYDROXYLASE FROM RAT-LIVER MITOCHONDRIA

被引：4

作者：

KARASZKIEWICZ, JW ^{[1
]}

KALF, GF ^{[1
]}

机构：

[1] THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT BIOCHEM & MOLEC BIOL,1020 LOCUST ST,PHILADELPHIA,PA 19107

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1035卷 / 02期

关键词：

(Rat liver); Benzene hydroxylase; Benzene metabolism; Mitochondrion; Phenol;

D O I：

10.1016/0304-4165(90)90121-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme has been purified to electrophoretic homogeneity from rat liver mitoplasts which metabolizes benzene to phenol. The enzyme has a Mr of 52 000 and requires NADPH, adrendoxin, and adrenodoxin reductase for activity, Benzene hydroxylase activity could be inhibited by carbon monoxide and SKF-525A, and by specific inhibitors of microsomal benzene metabolism. The purified enzyme also oxidized phenol to catechol. The data suggest that a cytochrome P-450 of microchondrial origin is involved in benzene metabolism, and provide another example of a role for the mitochondrion in xenobiotic activation. © 1990.

引用

页码：223 / 229

页数：7

共 29 条

[1] BENZENE-H-3 METABOLISM IN RABBIT BONE-MARROW [J].