BENZENE-H-3 METABOLISM IN RABBIT BONE-MARROW

被引：89

作者：

ANDREWS, LS

SASAME, HA

GILLETTE, JR

机构：

[1] NIGMS,PROGRAM PHARMACOL TOXICOL,BETHESDA,MD 20205

[2] NHLBI,CHEM PHARMACOL LAB,BETHESDA,MD 20205

来源：

LIFE SCIENCES | 1979年 / 25卷 / 07期

关键词：

D O I：

10.1016/0024-3205(79)90550-2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

An assay for benzene metabolism using 3H-benzene and high pressure liquid chromatography was developed. 3H-Benzene metabolism (2 pmoles benzene equivalents/mg protein/min) required the presence of a TPNH generating system and was inhibited 80% in the presence of a CO:O2 (9:1) atmosphere. The products of 3H-benzene rabbit bone marrow microsomal metabolism were phenol and an unidentified metabolite. Cytochrome P-450 (26-51 pmoles/mg microsomal protein) and cytochrome c reductase activity (7.8-21.0 nmole/mg microsomal protein/min) were detected in rabbit bone marrow. © 1979.

引用

页码：567 / 572

页数：6

共 23 条

[1]

AKSOY M, 1972, BRIT J IND MED, V28, P296

[2]

ANDREWS LS, 1976, RES COMMUN CHEM PATH, V15, P319

[3] EFFECTS OF TOLUENE ON METABOLISM, DISPOSITION AND HEMATOPOIETIC TOXICITY OF [BENZENE-H-3 [J].