COMPLEMENTATION OF A YEAST-CELL CYCLE MUTANT BY AN ALFALFA CDNA-ENCODING A PROTEIN-KINASE HOMOLOGOUS TO P34CDC2

被引：156

作者：

HIRT, H ^{[1
]}

PAY, A ^{[1
]}

GYORGYEY, J ^{[1
]}

BAKO, L ^{[1
]}

NEMETH, K ^{[1
]}

BOGRE, L ^{[1
]}

SCHWEYEN, RJ ^{[1
]}

HEBERLEBORS, E ^{[1
]}

DUDITS, D ^{[1
]}

机构：

[1] HUNGARIAN ACAD SCI,BIOL RES CTR,INST GENET,INST PLANT PATHOL,H-6701 SZEGED,HUNGARY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 05期

关键词：

PLANT CELL CYCLE; MEDICAGO-SATIVA L; PLANT CELL CULTURE; SOMATIC EMBRYOGENESIS; 2,4-DICHLOROPHENOXYACETIC ACID;

D O I：

10.1073/pnas.88.5.1636

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cdc2 protein kinase plays a central role in control of the eukaryotic cell cycle of animals and yeasts. We have isolated a cDNA clone (cdc2Ms) from alfalfa (Medicago sativa L.) that is homologous to the yeast cdc2/CDC28 genes. The encoded protein is 64% identical to the yeast and mammalian counterparts and shows all the prominent structural features known from these organisms. Antibody raised against a 16-amino acid synthetic peptide with crossreactivity against p34 proteins recognized a 34-kilodalton protein in extracts of alfalfa cells. When transferred into a fission yeast, the plant cdc2 homolog can complement a temperature-sensitive cdc2 mutant. Northern analysis revealed higher transcript levels in shoots and suspension cultures than in roots. In addition to the dominant transcript of 1.4 kilobases detected in the poly(A)+ fraction, 2.5- and 1.2-kilobase transcripts were detected in total RNA preparations from shoots or somatic embryos. Suspension cultures that were induced to form somatic embryos by an auxin (2,4-dichlorophenoxyacetic acid) showed fluctuations in transcription pattern during the induction period and embryogenesis.

引用

页码：1636 / 1640

页数：5

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