SEQUENCE-ANALYSIS OF THE HN GENE OF PARAINFLUENZA VIRUS TYPE-2

被引：16

作者：

PRECIOUS, B ^{[1
]}

SOUTHERN, JA ^{[1
]}

RANDALL, RE ^{[1
]}

机构：

[1] UNIV ST ANDREWS,DEPT BIOCHEM & MICROBIOL,N ST,ST ANDREWS KY16 9AL,FIFE,SCOTLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-5-1163

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cDNA library was constructed in λgt10 using mRNA purified from cells infected with parainfluenza virus type 2 (PIV2). Virus-specific clones were identified by screening the library with 32P-labelled cDNA probes made from randomly primed vRNA. Clones containing the haemagglutinin-neuraminidase (HN) gene were identified by sequence comparisons with known parainfluenza virus HN gene sequences. The largest HN clone isolated had a nucleic acid sequence of 2065 bp with a single long open reading frame encoding a protein of 571 amino acids. The HN protein has nine predicted glycosylation sites and an amino-terminal membrane-spanning region. The PIV2 HN protein shares 43% amino acid identity with the HN protein of simian virus 5 and 40% with mumps virus, 30% of the amino acids being common to all three viruses.

引用

页码：1163 / 1168

页数：6

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