REGULATION OF PHOSPHOFRUCTOKINASE ACTIVITY BY GLUCAGON IN ISOLATED RAT HEPATOCYTES

被引：68

作者：

PILKIS, S ^{[1
]}

SCHLUMPF, J ^{[1
]}

PILKIS, J ^{[1
]}

CLAUS, TH ^{[1
]}

机构：

[1] VANDERBILT UNIV,HOWARD HUGHES MED INST,LAB,NASHVILLE,TN 37232

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)91501-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucagon addition to isolated hepatocytes from fed rats resulted in an inhibition of the activity of phosphofructokinase measured in extracts of the cells. Glucagon caused a shift in the fructose 6-phosphate concentration curve to the right resulting in an increase in the K0.5 for F6P from 0.09 mM to 0.31 mM. No effect of glucagon was seen when the enzyme was assayed with saturating concentrations of fructose 6-phosphate or in the presence of 1 mM AMP. The effect of glucagon was seen within minutes and the concentration of hormone giving half-maximal inhibition was 0.2 nM. This effect of glucagon on phosphofructokinase activity may contribute to the effect of glucagon on substrate cycling at the fructose 6-phosphate-fructose bisphosphate level. © 1979.

引用

页码：960 / 967

页数：8

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