MAPPING OF COSMID CLONES IN HUNTINGTONS-DISEASE REGION OF CHROMOSOME-4

被引：51

作者：

WHALEY, WL

BATES, GP

NOVELLETTO, A

SEDLACEK, Z

CHENG, S

ROMANO, D

ORMONDROYD, E

ALLITTO, B

LIN, C

YOUNGMAN, S

BAXENDALE, S

BUCAN, M

ALTHERR, M

WASMUTH, J

WEXLER, NS

FRONTALI, M

FRISCHAUF, AM

LEHRACH, H

MACDONALD, ME

GUSELLA, JF

机构：

[1] IMPERIAL CANC RES FUND,LONDON WC2A 3PX,ENGLAND

[2] UNIV TOR VERGATA,DIPARTIMENTO BIOL,ROME,ITALY

[3] COLUMBIA UNIV,DEPT NEUROL,NEW YORK,NY 10032

[4] HEREDITARY DIS FDN,SANTA MONICA,CA 90401

[5] HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02114

[6] UNIV CALIF IRVINE,DEPT BIOL CHEM,IRVINE,CA 92717

[7] CNR,IST MED SPERIMENTALE,ROME,ITALY

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1991年 / 17卷 / 01期

关键词：

D O I：

10.1007/BF01233207

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Huntington's disease (HD) is tightly linked to genetic markers in 4p16.3. We have used a regional somatic cell hybrid mapping panel to isolate and map 25 cosmids to the proximal portion of 4p16.3 and 17 cosmids to the distal portion. The latter were positioned by long-range restriction mapping relative to previously mapped markers. One cosmid, L6 (D4S166), spans the critical breakpoint in the mapping panel that distinguishes proximal and distal 4p16.3. Four of the cosmids mapped distal to D4S90, the previous terminal marker on 4p, and stretched to within 75 kb of the telomere. Several of the cosmids that mapped between L6 and D4S90 were clustered near a number of previously isolated clones in a region with many NotI sites. Cosmid E4 (D4S168) was localized immediately proximal to the one remaining gap in the long-range restriction map of distal 4p16.3. Although pulsed field gel mapping with E4 failed to link the two segments of the map, the intervening gap was excluded as a potential site for the HD gene by genetic analysis.

引用

页码：83 / 91

页数：9

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