MALONDIALDEHYDE IS A BIOCHEMICAL MARKER OF PEROXIDATIVE DAMAGE IN THE ISOLATED REPERFUSED RAT-HEART

被引：36

作者：

DIPIERRO, D ^{[1
]}

TAVAZZI, B ^{[1
]}

LAZZARINO, G ^{[1
]}

GIARDINA, B ^{[1
]}

机构：

[1] UNIV ROME 2,CTR INTERUNIV STUDIO DANNO I POSSIA & IPEROSSIA,I-00173 ROME,ITALY

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1992年 / 116卷 / 1-2期

关键词：

MALONDIALDEHYDE; PEROXIDATIVE DAMAGE; REPERFUSED RAT HEART;

D O I：

10.1007/BF01270587

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Concentration of MDA in isolated control, ischemic, and reperfused rat hearts was determined by using a new sensitive and reproducible HPLC method on the perchloric acid extract of the freeze-clamped tissues. By means of this HPLC assay for the direct measurements of MDA, concentrations of adenine nucleotide derivatives were also obtained in the same chromatographic run. Under the present experimental conditions, no detectable amount of MDA could be observed in control hearts while ischemic hearts showed 0.009 mumoles/g d.w. of MDA (s.d. = 0.001), this value representing the sensitivity limit of the method employed. On the contrary, reperfused hearts showed 0.118 mumoles/g d.w. of MDA (s.d. = 0.036), thereby indicating that this compound originates from an oxygen free radical-mediated breakdown of phospholipids and demonstrating the existence of quantifiable molecular damage occurring upon reperfusion. On the whole, our data demonstrate that MDA, if properly assayed, is a reliable index of peroxidative injury to biological systems.

引用

页码：193 / 196

页数：4

共 23 条

[11]

KEHERER JP, 1987, FREE RADICAL RES COM, V3, P69

[12] CONCEPTS RELATED TO THE STUDY OF REACTIVE OXYGEN AND CARDIAC REPERFUSION INJURY [J].

KEHRER, JP .

FREE RADICAL RESEARCH COMMUNICATIONS, 1989, 5 (06) :305-314

[13] SIMULTANEOUS SEPARATION OF MALONDIALDEHYDE, ASCORBIC-ACID, AND ADENINE-NUCLEOTIDE DERIVATIVES FROM BIOLOGICAL SAMPLES BY ION-PAIRING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

LAZZARINO, G ;

DIPIERRO, D ;

TAVAZZI, B ;

CERRONI, L ;

GIARDINA, B .

ANALYTICAL BIOCHEMISTRY, 1991, 197 (01) :191-196

[14] A METHOD FOR PREPARING FREEZE-CLAMPED TISSUE SAMPLES FOR METABOLITE ANALYSES [J].

LAZZARINO, G ;

NUUTINEN, M ;

TAVAZZI, B ;

DIPIERRO, D ;

GIARDINA, B .

ANALYTICAL BIOCHEMISTRY, 1989, 181 (02) :239-241

[15] EVALUATION OF FREE-RADICAL AND LIPID PEROXIDE FORMATION DURING GLOBAL-ISCHEMIA AND REPERFUSION IN ISOLATED PERFUSED RAT-HEART [J].

MAUPOIL, V ;

ROCHETTE, L .

CARDIOVASCULAR DRUGS AND THERAPY, 1988, 2 (05) :615-621

[16] REAL-TIME CONTINUOUS-FLOW SPIN TRAPPING OF HYDROXYL FREE-RADICAL IN THE ISCHEMIC AND POST-ISCHEMIC MYOCARDIUM [J].

PIETRI, S ;

CULCASI, M ;

COZZONE, PJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 186 (1-2) :163-173

[17]

RECKNAGEL RO, 1984, METHOD ENZYMOL, V105, P331

[18] CONJUGATED DIENES IN ISCHEMIC AND REPERFUSED MYOCARDIUM - AN INVIVO CHEMICAL SIGNATURE OF OXYGEN FREE-RADICAL MEDIATED INJURY [J].

ROMASCHIN, AD ;

REBEYKA, I ;

WILSON, GJ ;

MICKLE, DAG .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1987, 19 (03) :289-302

[19]

ROTH E, 1985, BASIC RES CARDIOL, V80, P530

[20] OXYGEN RADICAL INJURY AND LOSS OF HIGH-ENERGY COMPOUNDS IN ANOXIC AND REPERFUSED RAT-HEART - PREVENTION BY EXOGENOUS FRUCTOSE-1,6-BISPHOSPHATE [J].

TAVAZZI, B ;

CERRONI, L ;

DIPIERRO, D ;

LAZZARINO, G ;

NUUTINEN, M ;

STARNES, JW ;

GIARDINA, B .

FREE RADICAL RESEARCH COMMUNICATIONS, 1990, 10 (03) :167-176

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