GENERATION AND ANALYSIS OF INTERLEUKIN-4 DEFICIENT MICE

被引：1146

作者：

KUHN, R

RAJEWSKY, K

MULLER, W

机构：

[1] Institute for Genetics, University of Cologne

来源：

SCIENCE | 1991年 / 254卷 / 5032期

关键词：

D O I：

10.1126/science.1948049

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Interleukin-4 (IL-4) promotes the growth and differentiation of many hematopoietic cells in vitro; in particular, it directs the immunoglobulin (Ig) class switch to IgG1 and IgE. Mice homozygous for a mutation that inactivates the IL-4 gene were generated to test the requirement for IL-4 in vivo. In the mutant mice T and B cell development was normal, but the serum levels of IgGl and IgE were strongly reduced. The IgG1 dominance in a T cell-dependent immune response was lost, and IgE was not detectable upon nematode infection. Thus, some but not all of the in vitro properties of IL-4 are critical for the physiology of the immune system in vivo.

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页码：707 / 710

页数：4

相关论文

共 39 条

[21] DISRUPTION OF THE PROTO-ONCOGENE INT-2 IN MOUSE EMBRYO-DERIVED STEM-CELLS - A GENERAL STRATEGY FOR TARGETING MUTATIONS TO NON-SELECTABLE GENES [J].

MANSOUR, SL ;

THOMAS, KR ;

CAPECCHI, MR .

NATURE, 1988, 336 (6197) :348-352

[22] HUMAN TH1 AND TH2 SUBSETS - DOUBT NO MORE - COMMENT [J].

MOSMANN, T .

IMMUNOLOGY TODAY, 1991, 12 (08) :257-257

[23]

MOSMANN TR, 1989, ANNU REV IMMUNOL, V7, P145, DOI 10.1146/annurev.iy.07.040189.001045

[24] MEMBRANE-BOUND IGM OBSTRUCTS B-CELL DEVELOPMENT IN TRANSGENIC MICE [J].

MULLER, W ;

RUTHER, U ;

VIEIRA, P ;

HOMBACH, J ;

RETH, M ;

RAJEWSKY, K .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1989, 19 (05) :923-928

[25] PRODUCTION OF A MONOCLONAL-ANTIBODY TO AND MOLECULAR CHARACTERIZATION OF B-CELL STIMULATORY FACTOR-I [J].

OHARA, J ;

PAUL, WE .

NATURE, 1985, 315 (6017) :333-336

[26] LOCALIZATION OF MURINE IG-1B AND IG-1A (IGG 2A) ALLOTYPIC DETERMINANTS DETECTED WITH MONOCLONAL ANTIBODIES [J].

OI, VT ;

HERZENBERG, LA .

MOLECULAR IMMUNOLOGY, 1979, 16 (12) :1005-1017

[27] STRUCTURAL-ANALYSIS OF THE MOUSE CHROMOSOMAL GENE ENCODING INTERLEUKIN-4 WHICH EXPRESSES B-CELL, T-CELL AND MAST-CELL STIMULATING ACTIVITIES [J].

OTSUKA, T ;

VILLARET, D ;

YOKOTA, T ;

TAKEBE, Y ;

LEE, F ;

ARAI, N ;

ARAI, K .

NUCLEIC ACIDS RESEARCH, 1987, 15 (01) :333-344

[28] B-CELL STIMULATORY FACTOR-1/INTERLEUKIN-4 [J].

PAUL, WE ;

OHARA, J .

ANNUAL REVIEW OF IMMUNOLOGY, 1987, 5 :429-459

[29] CLASS SWITCH RECOMBINATION IS IGG1 SPECIFIC ON ACTIVE AND INACTIVE IGH LOCI OF IGG1-SECRETING B-CELL BLASTS [J].

RADBRUCH, A ;

MULLER, W ;

RAJEWSKY, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) :3954-3957

[30] INTERLEUKIN-4 (B-CELL STIMULATORY FACTOR-I) CAN ENHANCE OR ANTAGONIZE THE FACTOR-DEPENDENT GROWTH OF HEMATOPOIETIC PROGENITOR CELLS [J].

RENNICK, D ;

YANG, G ;

MULLERSIEBURG, C ;

SMITH, C ;

ARAI, N ;

TAKABE, Y ;

GEMMELL, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (19) :6889-6893

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