SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF LIPID HYDROPEROXIDES

被引：164

作者：

HICKS, M

GEBICKI, JM

机构：

[1] School of Biological Sciences, Macquarie University, North Ryde

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 02期

关键词：

D O I：

10.1016/S0003-2697(79)80003-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modification of the iodine technique for peroxide determination is described, in which the reaction is carried out in deaerated methanol:acetic acid solution in a spectrophotometer cuvette. Measurements of the triiodide ions released are carried out either at 290 or 360 nm. In this solvent, the molar extinction coefficients are ε290 = 4.41 × 104 and ε360 = 2.80 × 104 liters mol-1 cm-1. Calibration with standard peroxide solutions showed that 1 mol of I3- is produced for every peroxide group added. In its present form, the method can be used to measure 1 nEq of lipid hydroperoxide. © 1979 Academic Press, Inc.

引用

页码：249 / 253

页数：5

共 23 条

[1]

AKASAWA T, 1978, J AM OIL CHEM SOC, V55, P619

[2] THE ABSORPTION SPECTRA OF I-2,I-3-,I-, IO3-,S4O6 AND S2O3= HEAT OF THE REACTION I-3-=I-2+I- [J].