A GENETIC TOOL USED TO IDENTIFY THIOREDOXIN AS A MEDIATOR OF A GROWTH INHIBITORY SIGNAL

被引：180

作者：

DEISS, LP ^{[1
]}

KIMCHI, A ^{[1
]}

机构：

[1] WEIZMANN INST SCI,DEPT MOLEC GENET & VIROL,IL-76100 REHOVOT,ISRAEL

来源：

SCIENCE | 1991年 / 252卷 / 5002期

关键词：

D O I：

10.1126/science.1901424

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Loss of sensitivity to growth inhibitory polypeptides is likely to be one of the events that participates in the formation of some tumors and might be caused by inactivation or loss of the genetic elements that transduce these extracellular signals. The isolation of such a gene was achieved by randomly inactivating genes by an anti-sense complementary DNA expression library followed by direct selection for growth in the presence of an inhibitory polypeptide. Thus, a gene whose inactivation conveyed growth resistance to interferon-gamma (IFN-gamma) was isolated. Sequence analysis showed complete identity with human thioredoxin, a dithiol reducing agent, implicated here in the IFN-gamma-mediated growth arrest of HeLa cells.

引用

页码：117 / 120

页数：4

共 53 条

[1] REDOX REGULATION OF FOS AND JUN DNA-BINDING ACTIVITY INVITRO [J].

ABATE, C ;

PATEL, L ;

RAUSCHER, FJ ;

CURRAN, T .

SCIENCE, 1990, 249 (4973) :1157-1161

[2] EXPRESSION AND PURIFICATION OF THE LEUCINE ZIPPER AND DNA-BINDING DOMAINS OF FOS AND JUN - BOTH FOS AND JUN CONTACT DNA DIRECTLY [J].

ABATE, C ;

LUK, D ;

GENTZ, R ;

RAUSCHER, FJ ;

CURRAN, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :1032-1036

[3] INHIBITION OF TUMOR-CELL GROWTH BY INTERFERON-GAMMA IS MEDIATED BY 2 DISTINCT MECHANISMS DEPENDENT UPON OXYGEN-TENSION - INDUCTION OF TRYPTOPHAN DEGRADATION AND DEPLETION OF INTRACELLULAR NICOTINAMIDE ADENINE-DINUCLEOTIDE [J].

AUNE, TM ;

POGUE, SL .

JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (03) :863-875

[4] MOLECULAR-CLONING AND COMPLETE AMINO-ACID-SEQUENCE OF FORM-I PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C [J].

BENNETT, CF ;

BALCAREK, JM ;

VARRICHIO, A ;

CROOKE, ST .

NATURE, 1988, 334 (6179) :268-270

[5] TERRITORIAL LIMITS AND FUNCTIONAL-ANATOMY OF THE SIMIAN VIRUS-40 REPLICATION ORIGIN [J].

BERGSMA, DJ ;

OLIVE, DM ;

HARTZELL, SW ;

SUBRAMANIAN, KN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (02) :381-385

[6]

BERNARD JA, 1989, P NATL ACAD SCI USA, V86, P1578

[7] NORMAL KERATINIZATION IN A SPONTANEOUSLY IMMORTALIZED ANEUPLOID HUMAN KERATINOCYTE CELL-LINE [J].

BOUKAMP, P ;

PETRUSSEVSKA, RT ;

BREITKREUTZ, D ;

HORNUNG, J ;

MARKHAM, A ;

FUSENIG, NE .

JOURNAL OF CELL BIOLOGY, 1988, 106 (03) :761-771

[8] INTERLEUKIN-1 PRETREATMENT DECREASES ISCHEMIA REPERFUSION INJURY [J].

BROWN, JM ;

WHITE, CW ;

TERADA, LS ;

GROSSO, MA ;

SHANLEY, PF ;

MULVIN, DW ;

BANERJEE, A ;

WHITMAN, GJR ;

HARKEN, AH ;

REPINE, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (13) :5026-5030

[9] ENHANCER-LIKE INTERFERON RESPONSIVE SEQUENCES OF THE HUMAN AND MURINE (2'-5') OLIGOADENYLATE SYNTHETASE GENE PROMOTERS [J].

COHEN, B ;

PERETZ, D ;

VAIMAN, D ;

BENECH, P ;

CHEBATH, J .

EMBO JOURNAL, 1988, 7 (05) :1411-1419

[10]

DEISS LP, UNPUB

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