CONTRAST TRANSFER FOR FROZEN-HYDRATED SPECIMENS .2. AMPLITUDE CONTRAST AT VERY LOW-FREQUENCIES

被引：41

作者：

TOYOSHIMA, C ^{[1
]}

YONEKURA, K ^{[1
]}

SASABE, H ^{[1
]}

机构：

[1] RIKEN,FRONTIER RES PROGRAM,WAKO,SAITAMA 35101,JAPAN

来源：

ULTRAMICROSCOPY | 1993年 / 48卷 / 1-2期

关键词：

D O I：

10.1016/0304-3991(93)90179-2

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The contrast transfer properties of frozen-hydrated specimens were examined quantitatively for low spatial frequencies (1/350-1/160 angstrom-1). Specimens used were tubular crystals of two membrane proteins, the acetylcholine receptor and calcium-ATPase. These specimens were found to behave as weak-phase-weak-amplitude objects. The contribution of amplitude contrast was 5.8% at 120 kV and 4.8% at 200 kV accelerating voltage. Combined with previous results, the level of amplitude contrast showed little dependence on the spatial frequency over the range examined so far (1/350-1/35 angstrom-1).

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页码：165 / 176

页数：12

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