MOLECULAR-CLONING AND CHROMOSOMAL LOCALIZATION OF A NOVEL HUMAN TRACHEOBRONCHIAL MUCIN CDNA CONTAINING TANDEMLY REPEATED SEQUENCES OF 48 BASE-PAIRS

被引：384

作者：

PORCHET, N

VANCONG, N

DUFOSSE, J

AUDIE, JP

GUYONNETDUPERAT, V

GROSS, MS

DENIS, C

DEGAND, P

BERNHEIM, A

AUBERT, JP

机构：

[1] INST GUSTAVE ROUSSY,CYTOGENET & GENET ONCOL LAB,CNRS ONCOL MOLEC,URA 1158,F-94805 VILLEJUIF,FRANCE

[2] INSERM,U124,F-59045 LILLE,FRANCE

[3] CHR LILLE,HOP C HURIEZ,BIOCHIM LAB,F-59037 LILLE,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91580-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A λgt11 cDNA library constructed from human tracheo-bronchial mucosa was screened with a polyclonal antiserum raised to chemically deglycosylated pronase glycopeptides from human bronchial mucins. Out of 20 positives clones, one partial cDNA clone was isolated and allowed to map a novel human tracheo-bronchial mucin gene. It contains 48 nucleotide tandem repeats quite perfectly identical which encodes a protein containing about 50% of hydroxy amino-acids. This clone hybridized to polydisperse messages produced by human tracheo-bronchial and human colonic mucosae. The gene (proposed name MUC 4) from which cDNA is derived maps to chromosome 3. © 1991.

引用

页码：414 / 422

页数：9

共 16 条

[11] THE HYPERVARIABLE GENE LOCUS PUM, WHICH CODES FOR THE TUMOR ASSOCIATED EPITHELIAL MUCINS, IS LOCATED ON CHROMOSOME-1, WITHIN THE REGION 1Q21-24 [J].

SWALLOW, DM ;

GENDLER, S ;

GRIFFITHS, B ;

KEARNEY, A ;

POVEY, S ;

SHEER, D ;

PALMER, RW ;

TAYLORPAPADIMITRIOU, J .

ANNALS OF HUMAN GENETICS, 1987, 51 :289-294

[12]

TIMPTE CS, 1988, J BIOL CHEM, V263, P1081

[13]

VANCONG N, 1986, ANN GENET-PARIS, V29, P20

[14]

VANCONG N, 1990, IN PRESS HUM GENET

[15] HUMAN EPITHELIAL TUMOR-ANTIGEN CDNA SEQUENCES - DIFFERENTIAL SPLICING MAY GENERATE MULTIPLE PROTEIN FORMS [J].

WRESCHNER, DH ;

HAREUVENI, M ;

TSARFATY, I ;

SMORODINSKY, N ;

HOREV, J ;

ZARETSKY, J ;

KOTKES, P ;

WEISS, M ;

LATHE, R ;

DION, A ;

KEYDAR, I .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 189 (03) :463-473

[16]

1989, CYTOGENET CELL GENET, V51, P1

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