FIBROBLAST GROWTH FACTOR-I INDUCES PHOSPHOFRUCTOKINASE, FATTY-ACID SYNTHASE AND CA2+-ATPASE MESSENGER-RNA EXPRESSION IN NIH 3T3 CELLS

被引：42

作者：

HSU, DKW ^{[1
]}

DONOHUE, PJ ^{[1
]}

ALBERTS, GF ^{[1
]}

WINKLES, JA ^{[1
]}

机构：

[1] AMER RED CROSS,HOLLAND LAB,DEPT MOLEC BIOL,15601 CRABBS BRANCH WAY,ROCKVILLE,MD 20855

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.2644

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polypeptide growth factors act in part by inducing the expression of specific proteins that perform functions critical to cell cycle progression. We have used a differential display technique to identify genes that are expressed at higher levels following fibroblast growth factor (FGF)-1 (acidic FGF) stimulation of quiescent murine NIH 313 fibroblasts. Three such genes - liver (B-type) phosphofructokinase (PFK), fatty acid synthase (FAS) and sarco(endo)plasmic reticulum Ca2+-ATPase type 2 (SERCA2) - are described in this report. The level of FAS and SERCA2 mRNA expression is increased rapidly after FGF-1 addition; in contrast, PFK mRNA is induced with kinetics more typical of delayed-early genes. These results indicate that enhanced expression of the PFK, FAS and SERCA2 proteins may be important for FGF-1-stimulated cell proliferation. © 1993 Academic Press, Inc.

引用

页码：1483 / 1491

页数：9