EXPRESSION OF MAMMALIAN RENAL TRANSPORTERS IN XENOPUS-LAEVIS OOCYTES

被引：25

作者：

COADY, MJ ^{[1
]}

PAJOR, AM ^{[1
]}

TOLOZA, EM ^{[1
]}

WRIGHT, EM ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT PHYSIOL,LOS ANGELES,CA 90024

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1990年 / 283卷 / 01期

关键词：

D O I：

10.1016/0003-9861(90)90622-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have injected mRNA from rabbit renal cortex into Xenopus oocytes and demonstrated the expression of renal carriers for Na+-independent transport of l-phenylalanine and l-lysine and Na+-dependent transport of l-alanine and succinate. Maximal expression of renal amino acid transporters occurred 6-8 days following mRNA injection. The proteins responsible for transport of these four substrates were translated from mRNAs which are between 1.5 and 3.0 kb. This information serves as a starting point for expression cloning of these transport proteins. © 1990.

引用

页码：130 / 134

页数：5

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