CSF PRETREATMENT AND THE DIAGNOSIS OF HERPES-ENCEPHALITIS USING THE POLYMERASE CHAIN-REACTION

被引：39

作者：

DENNETT, C ^{[1
]}

KLAPPER, PE ^{[1
]}

CLEATOR, GM ^{[1
]}

LEWIS, AG ^{[1
]}

机构：

[1] UNIV MANCHESTER, SCH MED, DEPT PATHOL SCI, DIV VIROL, OXFORD RD, MANCHESTER M13 9PT, LANCS, ENGLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 34卷 / 01期

关键词：

HERPES ENCEPHALITIS; PCR; HERPESVIRUS SIMPLEX; CEREBROSPINAL FLUID; CSF; PRETREATMENT;

D O I：

10.1016/0166-0934(91)90125-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A number of techniques for extraction of DNA prior to polymerase chain reaction (PCR) amplification of herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF) were compared to the use of "native" CSF in the PCR reaction. The results indicate that extraction of DNA (which allows efficient removal of inhibitors of Taq polymerase) is an essential pre-requisite of the PCR detection of CSF HSV DNA.

引用

页码：101 / 104

页数：4

共 15 条

[1] RAPID DIAGNOSIS OF HERPES-SIMPLEX ENCEPHALITIS BY NESTED POLYMERASE CHAIN-REACTION ASSAY OF CEREBROSPINAL-FLUID [J].