DIAGNOSIS OF HERPES-ENCEPHALITIS VIA SOUTHERN BLOTTING OF CEREBROSPINAL-FLUID DNA AMPLIFIED BY POLYMERASE CHAIN-REACTION

被引：67

作者：

KLAPPER, PE

CLEATOR, GM

DENNETT, C

LEWIS, AG

机构：

[1] Department of Pathological Sciences, Division of Virology, University of Manchester, Manchester

来源：

JOURNAL OF MEDICAL VIROLOGY | 1990年 / 32卷 / 04期

关键词：

THYMIDINE KINASE; NONINVASIVE DIAGNOSIS; HERPES SIMPLEX VIRUS;

D O I：

10.1002/jmv.1890320413

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumbar cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR-SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant "non-invasive" method for the diagnosis of HSE.

引用

页码：261 / 264

页数：4

共 28 条

[1] POLYMERASE CHAIN-REACTION AND VIRAL CULTURE TECHNIQUES TO DETECT HSV IN SMALL VOLUMES OF CEREBROSPINAL-FLUID - AN EXPERIMENTAL MOUSE ENCEPHALITIS STUDY [J].