A NEW AND RAPID METHOD FOR CLINICAL DETERMINATION OF ALPHA-AMYLASE ACTIVITIES IN HUMAN SERUM AND URINE . OPTIMAL CONDITIONS

被引：758

作者：

CESKA, M

BIRATH, K

BROWN, B

机构：

[1] Department of Biochemistry, Pharmacia AB

来源：

CLINICA CHIMICA ACTA | 1969年 / 26卷 / 03期

关键词：

D O I：

10.1016/0009-8981(69)90071-0

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A new type of α-amylase starch substrate rendered insoluble by cross-linking and coloured by a dye marker, was shown to be hydrolyzed by α-amylase from Hyland standard serum, Versatol-E and Enzatrol. The various conditions for enzymatic hydrolysis of such a blue starch polymer were studied. It was possible, with considerable accuracy, to determine α-amylase over more than a thousandfold range of enzyme concentration. It is felt that such a novel substrate may assume a unique importance for determination of α-amylase in various human body fluids. © 1969.

引用

页码：437 / &