A NEW AND RAPID METHOD FOR CLINICAL DETERMINATION OF ALPHA-AMYLASE ACTIVITIES IN HUMAN SERUM AND URINE . OPTIMAL CONDITIONS

被引：758

作者：

CESKA, M

BIRATH, K

BROWN, B

机构：

[1] Department of Biochemistry, Pharmacia AB

来源：

CLINICA CHIMICA ACTA | 1969年 / 26卷 / 03期

关键词：

D O I：

10.1016/0009-8981(69)90071-0

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A new type of α-amylase starch substrate rendered insoluble by cross-linking and coloured by a dye marker, was shown to be hydrolyzed by α-amylase from Hyland standard serum, Versatol-E and Enzatrol. The various conditions for enzymatic hydrolysis of such a blue starch polymer were studied. It was possible, with considerable accuracy, to determine α-amylase over more than a thousandfold range of enzyme concentration. It is felt that such a novel substrate may assume a unique importance for determination of α-amylase in various human body fluids. © 1969.

引用

页码：437 / &

共 13 条

[1] A NEW METHOD FOR DETERMINATION OF ALPHA-AMYLASE [J].

CESKA, M ;

HULTMAN, E ;

INGELMAN, BG .

EXPERIENTIA, 1969, 25 (05) :555-&

[2] AN ULTRA-MICRO METHOD FOR DETERMINATION OF AMYLASE ACTIVITY IN BLOOD SERUM [J].

CLOSE, JR ;

STREET, HV .

CLINICA CHIMICA ACTA, 1958, 3 (05) :476-479

[3]

DAVISON WC, 1925, B J HOPKINS HOSP, V37, P281

[4]

ELMAN ROBERT, 1927, ARCH INTERNAL MED, V40, P58

[5]

GUTH PH, 1960, AM J GASTROENTEROL, V33, P139

[6]

HENRY RJ, 1960, CLIN CHEM, V6, P434

[7] COLORIMETRIC DETERMINATION OF AMYLASE [J].

HUGGINS, C ;

RUSSELL, PS .

ANNALS OF SURGERY, 1948, 128 (04) :668-678

[8]

PERALTA O, 1955, CLIN CHEM, V1, P157

[9] A NEW METHOD FOR DETERMINATION OF ALPHA-AMYLASE [J].

RINDERKNECHT, H ;

WILDING, P ;

HAVERBACK, BJ .

EXPERIENTIA, 1967, 23 (10) :805-+

[10] DETERMINATION OF AMYLASE ACTIVITY IN BIOLOGICAL FLUIDS [J].

STREET, HV ;

CLOSE, JR .

CLINICA CHIMICA ACTA, 1956, 1 (03) :256-268

← 1 2 →