HETERODIMERIZATION OF THE IL-2 RECEPTOR BETA-CHAIN AND GAMMA-CHAIN CYTOPLASMIC DOMAINS IS REQUIRED FOR SIGNALING

THE interaction of interleukin-2 (IL-2) and IL-2 receptors critically regulates the T-cell immune response following antigen activation(1,2). IL-2 can signal through high or intermediate affinity receptors which contain IL-2R alpha (refs 3, 4) + beta (refs 5-8) + gamma (ref. 9) or beta + gamma chains, respectively. IL-2R gamma is a common gamma chain, gamma(c), also shared by the IL-7 (ref. 10) and IL-4 (refs 11, 12) receptors, which when mutated results in X-linked severe combined immunodeficiency(13). Using chimaeric receptor constructs together with monoclonal or bispecific antibodies we demonstrate here that IL-2 signalling requires ligand-induced extracellular-domain-mediated heterodimerization of the beta- and gamma(c)-chain cytoplasmic domains. Anti-IL-2R alpha monoclonal antibodies trigger proliferation of cells transfected with chimaeric constructs in which the extracellular domains of IL-2R beta and gamma(c), are replaced by that of IL-2R alpha. Other experiments using chimaeric constructs indicated that IL-2 binds monomerically and monovalently to IL-2R alpha and that the beta-transmembrane domain is not required for receptor chain interactions. Finally, we provide a method for mapping residues in the gamma(c) cytoplasmic domain even in cells that constitutively express gamma(c).