CHARACTERIZATION OF MOUSE RIBOSOMAL GENE FRAGMENTS PURIFIED BY MOLECULAR CLONING

被引：146

作者：

ARNHEIM, N

机构：

[1] Biochemistry Department, State University of New York, Stony Brook

来源：

GENE | 1979年 / 7卷 / 02期

基金：

美国国家科学基金会;

关键词：

BamHI); Eco; PstI; Recombinant DNA; restriction endonucleases; RI; SalI; λgtWES vector;

D O I：

10.1016/0378-1119(79)90025-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Four mouse ribosomal gene fragments cloned in λgtWES were studied by restriction enzyme mapping and Southern transfer experiments. These fragments were found to contain 18S DNA and transcribed as well as non-transcribed spacer DNA. Variation in the structure of these mouse DNA inserts was limited to one region of spacer DNA. This variation may reflect real structural differences found in mouse ribosomal genes or possibly deletion events which occurred during cloning. The transcribed regions of the inserts appear identical to one another and restriction enzyme fragments from this region correspond to fragments observed in digests of total mouse DNA. These clones will be useful in studying the structure of transcribed spacer DNA including the ribosomal gene promoter. © 1979.

引用

页码：83 / 96

页数：14

共 26 条

[21] PURIFICATION AND CLONING OF A MOUSE RIBOSOMAL GENE FRAGMENT IN COLIPHAGE LAMBDA [J].