ISOLATION OF 3 NOVEL HUMAN GENES ENCODING G-PROTEIN-COUPLED RECEPTORS

被引:90
作者
HEIBER, M
DOCHERTY, JM
SHAH, G
NGUYEN, T
CHENG, R
HENG, HHQ
MARCHESE, A
TSUI, LC
SHI, XM
GEORGE, SR
ODOWD, BF
机构
[1] UNIV TORONTO, DEPT PHARMACOL, TORONTO, ON M5S 1A8, CANADA
[2] UNIV TORONTO, DEPT MED, TORONTO, ON M5S 1A8, CANADA
[3] HOSP SICK CHILDREN, RES INST, DEPT GENET, TORONTO, ON M5G 1X8, CANADA
[4] ADDICT RES FDN, TORONTO, ON M5S 2S1, CANADA
关键词
D O I
10.1089/dna.1995.14.25
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have cloned and mapped the chromosomal location of three novel human genes encoding G protein-coupled receptors that we have named GPR6, GPR5, and GPR4. The entire coding region for each of these genes was contained on single exons, Gene GPR6 encoded a receptor that shared closest identity (71% in the transmembrane regions) with the human orphan receptor GPR3 and was localized to chromosome 6 (q21-q22.1). Northern blot analysis revealed that GPR6 transcripts were abundant in the human putamen and to a lesser extent in the frontal cortex, hippocampus, and hypothalamus. Gene GPR5 encoded a receptor that most closely resembled the orphan receptor RBS11 (48% in the transmembrane regions) and the MIP 1 alpha/RANTES receptor (45% in the transmembrane regions) and was localized to chromosome 3 (p21.3-p21.1). Gene GPR4 shared identity (40% in the transmembrane regions) with the human platelet-activating factor receptor and was localized to chromosome 19 (q13.2-q13.3).
引用
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页码:25 / 35
页数:11
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