LINKAGE ANALYSES IN BRITISH PEDIGREES SUGGEST A SINGLE-LOCUS FOR DARIER DISEASE AND NARROW THE LOCATION TO THE INTERVAL BETWEEN D12S105 AND D12S129

Darier disease is a dominantly inherited skin disorder in which there appears to be abnormal adhesion between keratinocytes. We and others have shown that the disease in some British pedigrees is closely linked to markers mapping to 12q23-q24.1. In the present study we have defined crossovers that enable us to narrow the location of the disease gene to the interval between the D12S105 and the D12S129 markers. This interval may be expected to be on the order of about 4 cM on the basis of linkage data obtained using the primary CEPH reference families. Our data provide further evidence for locus homogeneity: each of four large British pedigrees, two of which have previously been subjected to preliminary characterization, shows statistically significant evidence for linkage to markers mapping to 12q23-q24.1. (C) 1994 Academic Press, Inc.