ISOELECTRIC POINTS OF PROTEINS, DETERMINED BY ISOELECTRIC-FOCUSING IN THE PRESENCE OF UREA AND ETHANOL

被引：43

作者：

GELSEMA, WJ

DELIGNY, CL

VANDERVEEN, NG

机构：

[1] Analytical Chemistry Laboratory, State University Utrecht, Utrecht

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 171卷 / APR期

关键词：

D O I：

10.1016/S0021-9673(01)95297-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Isoelectric points, pIapp, in sucrose-urea-water and glycerol-ethanol-water mixtures and isoelectric points, pI, in water have been determined at 25° for some carrier ampholytes. The differences, pIapppI, are shown to account for the primary medium effect and the pH measuring cell effect on the isoelectric point. The diferences, pIapppI, for Ampholines are used to correct apparent isoelectric points of proteins. pI shifts resulting from the denaturing effect of urea and ethanol are discussed in terms of the conformation change. © 1979.

引用

页码：171 / 181

页数：11