ALTERNATIVE SPLICING GENERATES 2 ISOFORMS OF THE ALPHA-2 SUBUNIT OF THE INHIBITORY GLYCINE RECEPTOR

被引：117

作者：

KUHSE, J

KURYATOV, A

MAULET, Y

MALOSIO, ML

SCHMIEDEN, V

BETZ, H

机构：

[1] MAX PLANCK INST BRAIN RES,DEUTSCHORDENSTR 46,W-6000 FRANKFURT,GERMANY

[2] UNIV HEIDELBERG,ZENTRUM MOLEK BIOL HEIDELBERG,W-6900 HEIDELBERG,GERMANY

来源：

FEBS LETTERS | 1991年 / 283卷 / 01期

关键词：

GLYCINE RECEPTOR; ALTERNATIVE SPLICING; RECEPTOR HETEROGENEITY; BRAIN DEVELOPMENT;

D O I：

10.1016/0014-5793(91)80557-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha-2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced protein sequences (alpha-2A and alpha-2B) exhibit 99% identity with the previously characterized human alpha-2 subunit. In situ hybridization revealed expression of both alpha-2A and alpha-2B mRNAs in the prenatal rat brain, suggesting that these variant proteins may have a role in synaptogenesis. Heterologous expression in Xenopus oocytes showed that the more abundantly expressed alpha-2A subunit forms strychnine-sensitive ion channels which resemble human alpha-2 subunit GlyRs in their electrophysiological properties.

引用

页码：73 / 77

页数：5

共 22 条

[11] ALPHA-SUBUNIT VARIANTS OF THE HUMAN GLYCINE RECEPTOR - PRIMARY STRUCTURES, FUNCTIONAL EXPRESSION AND CHROMOSOMAL LOCALIZATION OF THE CORRESPONDING GENES [J].