EFFECT OF SULFATED POLYSACCHARIDES AND SULFATE ANIONS ON THE AMP-DEPENDENT ACTIVITY OF PHOSPHORYLASE-B

被引：14

作者：

SOTIROUDIS, TG

OIKONOMAKOS, NG

EVANGELOPOULOS, AE

机构：

[1] The National Hellenic Research Foundation, Athens 501/1

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91615-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of sulfated polysaccharides on the AMP-dependent activity of rabbit muscle phosphorylase b as compared with that of Na2SO4 has been studied. It has been shown that sulfated polysaccharides and Na2SO4 greatly stimulated AMP-activation of the enzyme at low AMP concentrations. Dextran sulfate and Na2SO4 desensitized the allosteric interactions of the enzyme towards the nucleotide activator and reversed the enzyme inhibition caused by glucose-6-phosphate and glucose. Furthermore, it was found that while dextran sulfate decreased the Km value for both substrates, glucose-1-phosphate and glycogen, sulfate anions decreased only the Km value for glycogen. © 1979.

引用

页码：234 / 239

页数：6

共 19 条

[11]

OIKONOMAKOS NG, BIOCH J

[12] REGULATION OF RAT PLACENTAL PHOSPHORYLASE [J].

ROSS, E ;

WALSH, D .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 264 (03) :490-+

[13]

SATO K, 1973, CANCER RES, V33, P724

[14]

SILONOVA GV, 1967, DOKL AKAD NAUK SSSR+, V174, P718

[15] EFFECT OF POLYCARBOXYLATES ON PHOSPHORYLASE-B [J].

SOTIROUDIS, TG ;

OIKONOMAKOS, NG ;

EVANGELOPOULOS, AE .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 86 (03) :674-682

[16] STIMULATION OF LIVER PHOSPHORYLASE-B BY AMP, FLUORIDE AND SULFATE - TECHNICAL NOTE ON SPECIFIC DETERMINATION OF A AND B FORMS OF LIVER-GLYCOGEN PHOSPHORYLASE [J].

STALMANS, W ;

HERS, HG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 54 (02) :341-350

[17]

VIKTOROVA LN, 1975, DOKL AKAD NAUK SSSR+, V222, P1463

[18]

Wang J. H, 1972, ENZYMES, V7, P435

[19]

WANG JH, 1970, J BIOL CHEM, V245, P176

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