CLONING OF RAT OBESE CDNA AND ITS EXPRESSION IN OBESE RATS

被引：153

作者：

MURAKAMI, T ^{[1
]}

SHIMA, K ^{[1
]}

机构：

[1] UNIV TOKUSHIMA,SCH MED,DEPT LAB MED,TOKUSHIMA 770,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 209卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1589

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mouse obese gene product, expressed specifically in adipose tissue, regulates energy balance in mice. Mutation of the obese gene results in marked obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Here we report the cloning and sequencing of rat obese cDNA. Neither alterations of nucleotide sequence in the coding region nor changes of the gene structure were found in two rat strains with obesity, Zucker (fa/fa) and Otsuka Long Evans Tokushima Fatty. The expression level of obese mRNA in adipose tissue of Zucker (fa/fa) rat was found to be about 4 times that in lean littermates, suggesting some mutation or abnormal expression of the receptor for the obese product in obese rats of this strain. (C) 1995 Academic Press, Inc.

引用

页码：944 / 952

页数：9

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