THE JELLYFISH GREEN FLUORESCENT PROTEIN - A NEW TOOL FOR STUDYING ION-CHANNEL EXPRESSION AND FUNCTION

被引：220

作者：

MARSHALL, J

MOLLOY, R

MOSS, GWJ

HOWE, JR

HUGHES, TE

机构：

[1] YALE UNIV,SCH MED,NEUROBIOL SECT,NEW HAVEN,CT 06520

[2] YALE UNIV,SCH MED,DEPT OPHTHALMOL & VISUAL SCI,NEW HAVEN,CT 06520

来源：

NEURON | 1995年 / 14卷 / 02期

关键词：

D O I：

10.1016/0896-6273(95)90279-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Two methods are described for using the jellyfish green fluorescent protein (GFB) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing.

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页码：211 / 215

页数：5

相关论文

共 16 条

[1] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[2] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].

CHEN, C ;

OKAYAMA, H .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752

[3]

Colquhoun Colquhoun D. D., 1983, SINGLE CHANNEL RECOR, P191

[4] CLONING OF A CDNA FOR A GLUTAMATE RECEPTOR SUBUNIT ACTIVATED BY KAINATE BUT NOT AMPA [J].

EGEBJERG, J ;

BETTLER, B ;

HERMANSBORGMEYER, I ;

HEINEMANN, S .

NATURE, 1991, 351 (6329) :745-748

[5] CHARACTERISTICS OF A HUMAN CELL LINE TRANSFORMED BY DNA FROM HUMAN ADENOVIRUS TYPE-5 [J].

GRAHAM, FL ;

SMILEY, J ;

RUSSELL, WC ;

NAIRN, R .

JOURNAL OF GENERAL VIROLOGY, 1977, 36 (JUL) :59-72

[6] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[7] MOLECULAR DIVERSITY OF THE NMDA RECEPTOR CHANNEL [J].

KUTSUWADA, T ;

KASHIWABUCHI, N ;

MORI, H ;

SAKIMURA, K ;

KUSHIYA, E ;

ARAKI, K ;

MEGURO, H ;

MASAKI, H ;

KUMANISHI, T ;

ARAKAWA, M ;

MISHINA, M .

NATURE, 1992, 358 (6381) :36-41

[8] HETEROMERIC NMDA RECEPTORS - MOLECULAR AND FUNCTIONAL DISTINCTION OF SUBTYPES [J].

MONYER, H ;

SPRENGEL, R ;

SCHOEPFER, R ;

HERB, A ;

HIGUCHI, M ;

LOMELI, H ;

BURNASHEV, N ;

SAKMANN, B ;

SEEBURG, PH .

SCIENCE, 1992, 256 (5060) :1217-1221

[9] MOLECULAR-CLONING AND CHARACTERIZATION OF THE RAT NMDA RECEPTOR [J].

MORIYOSHI, K ;

MASU, M ;

ISHII, T ;

SHIGEMOTO, R ;

MIZUNO, N ;

NAKANISHI, S .

NATURE, 1991, 354 (6348) :31-37

[10]

MOSS GWJ, 1995, IN PRESS BIOPHYS J