ISOLATION OF AN AMINOGLYCOSIDE RECEPTOR FROM GUINEA-PIG INNER-EAR TISSUES AND KIDNEY

被引：126

作者：

SCHACHT, J

机构：

[1] Kresge Hearing Research Institute, The University of Michigan, Ann Arbor, 48109, MI

来源：

ARCHIVES OF OTO-RHINO-LARYNGOLOGY-ARCHIV FUR OHREN-NASEN-UND KEHLKOPFHEILKUNDE | 1979年 / 224卷 / 1-2期

关键词：

Affinity chromatography; Aminoglycoside ototoxicity; Drug receptors; Neomycin; Polyphosphoinositides;

D O I：

10.1007/BF00455236

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Affinity chromatography was used to isolate aminoglycoside receptors from inner ear tissues and kidney. Neomycin was immobilized on glass beads and served as the stationary phase in column chromatography. Fractionation of an organic tissue extract on this matrix demonstrated two components with high affinity for neomycin: phosphatidyl inositol phosphate and phosphatidyl inositol diphosphate. The toxicity of aminoglycosides is explained on the basis of a drug-interaction with these lipids. © 1979 Springer-Verlag.

引用

页码：129 / 134

页数：6