IRON-DEXTRAN ANTIBODY CONJUGATES - GENERAL-METHOD FOR SIMULTANEOUS STAINING OF 2 COMPONENTS IN HIGH-RESOLUTION IMMUNOELECTRON MICROSCOPY

被引：71

作者：

DUTTON, AH

TOKUYASU, KT

SINGER, SJ

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 07期

关键词：

D O I：

10.1073/pnas.76.7.3392

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the preparation and properties of an electron-dense antibody conjugate, made by the covalent bonding of an iron-dextran (Imposil) particle to an antibody molecule. Transmission electron microscopic experiments with the Imposil-antibody conjugates demonstrate their suitability as specific immunostains at high resolution, particularly for simultaneous double staining experiments in conjunction with ferritin-antibody conjugates.

引用

页码：3392 / 3396

页数：5

共 26 条

[21] PROPERTIES OF SPECIFIC STAINS FOR ELECTRON MICROSCOPY PREPARED BY CONJUGATION OF ANTIBODY MOLECULES WITH FERRITIN [J].