THE PROTEIN ID - A NEGATIVE REGULATOR OF HELIX-LOOP-HELIX DNA-BINDING PROTEINS

被引：2056

作者：

BENEZRA, R ^{[1
]}

DAVIS, RL ^{[1
]}

LOCKSHON, D ^{[1
]}

TURNER, DL ^{[1
]}

WEINTRAUB, H ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT PATHOL,SEATTLE,WA 98195

来源：

CELL | 1990年 / 61卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0092-8674(90)90214-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a cDNA clone encoding a novel helix-loop-helix (HLH) protein, Id. Id is missing the basic region adjacent to the HLH domain that is essential for specific DNA binding in another HLH protein, MyoD. An in vitro translation product of Id can associate specifically with at least three HLH proteins (MyoD, E12, and E47) and attenuate their ability to bind DNA as homodimeric or heterodimeric complexes. Id is expressed at varying levels in all cell lines tested. In three cell lines that can be induced to undergo terminal differentiation, Id RNA levels decrease upon induction. Transfection experiments indicate that over-expression of Id inhibits the trans-activation of the muscle creatine kinase enhancer by MyoD. Based on these findings, we propose that HLH proteins lacking a basic region may negatively regulate other HLH proteins through the formation of nonfunctional heterodimeric complexes. © 1990.

引用

页码：49 / 59

页数：11

共 58 条

[21] RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS [J].