A RAPID, AUTOMATED MICROTITER ASSAY FOR MEASURING NEUTRALIZATION OF HIV-1

被引：18

作者：

LAAL, S

BURDA, S

SHARPE, S

ZOLLAPAZNER, S

机构：

[1] VET AFFAIRS MED CTR,423 E 23RD ST,NEW YORK,NY 10010

[2] NYU MED CTR,NEW YORK,NY 10016

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1993年 / 9卷 / 08期

关键词：

D O I：

10.1089/aid.1993.9.781

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A sensitive, rapid, and quantative ELISA for p24 is described, which can be used as the read-out to test for HIV-1 neutralization in the syncytium-forming microassay and can replace the counting of syncytia under the microscope. This assay can be used reliably for divergent strains of HIV-1, including those that do not induce syncytia. The new read-out permits evaluation of neutralization at 3 days rather than 5 days. Using this assay, the neutralizing activity of several new and previously described human monoclonal antibodies against HIV-1 was characterized.

引用

页码：781 / 785

页数：5