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INTEGRATION AND TRANSCRIPTION OF MOUSE MAMMARY-TUMOR VIRUS-DNA IN RAT HEPATOMA-CELLS

被引：117
作者：
RINGOLD, GM
SHANK, PR
VARMUS, HE
RING, J
YAMAMOTO, KR
机构：
[1] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143
[2] UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL,SAN FRANCISCO,CA 94143
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 02期
关键词：
D O I：
10.1073/pnas.76.2.665
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Rat hepatoma cells infected with mouse mammary tumor virus (MMTV) acquire viral DNA that becomes covalently linked to the cell DNA. Using restriction endonucleases and the DNA transfer procedure of Southern [Southern, E.M. (1975) J. Mol. Biol. 98, 503-517], we have studied the sites in cellular DNA into which MMTV DNA inserts. These experiments indicate that: (i) there are many sites in cell DNA into which MMVA DNA integrates; (ii) the junctions between viral and cellular DNA occur within a limited portion of the viral genome; (iii) clones that contain MMTV DNA do not necessarily produce viral RNA; and (iv) the extent of transcription and glucocorticoid responsiveness of MMTV proviruses may be dependent on the site(s) in cell DNA in which the viral DNA resides.
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页码：665 / 669
页数：5
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