NUCLEOTIDE-SEQUENCE OF THE GENE CODING FOR THE BANIII DNA METHYLTRANSFERASE IN BACILLUS-ANEURINOLYTICUS

被引：7

作者：

KAWAKAMI, B

SASAKI, A

OKA, M

MAEKAWA, Y

机构：

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 12期

关键词：

D O I：

10.1080/00021369.1990.10870493

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The gene coding for the ATCGAT specific BanIII DNA methyltransferase (M-BanIII) of Bacillus aneurinolyticus was cloned and its nucleotides sequenced. The coding region was assigned on the nucleotide sequence on the basis of the N-terminal amino acid sequence and molecular weight of the enzyme. The M-BanIII gene coded for a protein of 580 amino acid residues (MW 66,344). Comparison with other methylases indicated that the M-BanIII sequence contained a segment of tetra-amino acids, NPPY, characteristic of N6-adenine methylases. In addition, some homologous regions were found in the sequences of type II adenine methylases PaeR71(CTCGAG), TaqI(TCGA) and PstI(CTGCAG), containing TCGA within the recognition sequences.

引用

页码：3227 / 3233

页数：7

共 20 条

[1] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].