PURIFICATION OF HUMAN PLATELET-DERIVED GROWTH-FACTOR

被引：598

作者：

ANTONIADES, HN

SCHER, CD

STILES, CD

机构：

[1] SIDNEY FARBER CANC INST,DEPT HEMATOL ONCOL,BOSTON,MA 02115

[2] CHILDRENS HOSP,BOSTON,MA 02115

[3] HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115

[4] SIDNEY FARBER CANC INST,TUMOR BIOL LAB,GRP W,BOSTON,MA 02115

[5] HARVARD UNIV,SCH PUBL HLTH,DEPT NUTR,BOSTON,MA 02115

[6] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 04期

关键词：

D O I：

10.1073/pnas.76.4.1809

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human platelets cotain a polypeptide growth factor that stimulates the proliferation of connective tissue cells. Purification of this platelet-derived growth factor (PDGF) was accomplished by heat (100°C) treatment of washed platelets and subsequent ion-exchange chromatography, gel filtration in 1 M acetic acid, isoelectric focusing, and preparative sodium dodecyl sulfate/polyacrylamide gel electrophoresis. PDGF has an isoelectric point of 9.8 and a molecular weight ranging from 13,000 to 16,000 as judged by gel filtration in 1 M acetic acid or analytical sodium dodecyl sulfate gel electrophoresis under reducing conditions. The specific activity of the purified PDGF is 20 million times greater than that found in unfractionated human serum. Purified PDGF stimulates replicative DNA synthesis and cell proliferation in quiescent density-arrested cultures of BALB/c 3T3 cells at concentrations of 1 ng/ml (0.1 nM).

引用

页码：1809 / 1813

页数：5

共 28 条

[1] RADIOIMMUNOASSAY OF A HUMAN-SERUM GROWTH-FACTOR FOR BALB-C-3T3 CELLS - DERIVATION FROM PLATELETS [J].