EARLY EMBRYONIC-DEVELOPMENT OF THE GRAY SHORT-TAILED OPOSSUM, MONODELPHIS-DOMESTICA, INVIVO AND INVITRO

被引:57
作者
BAGGOTT, LM [1 ]
MOORE, HDM [1 ]
机构
[1] AFRC,MRC,ZOOL SOC LONDON,INST ZOOL,COMPARAT PHYSIOL RES GRP,LONDON NW1 4RY,ENGLAND
关键词
D O I
10.1111/j.1469-7998.1990.tb06019.x
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Eggs and embryos (up to the unilaminar blastocyst stage) were collected from grey short-tailed opossums, Monodelphis domestica, at known time intervals after mating. Some were fixed immediately for examination by light and electron microscopy while others were maintained in culture at 37-degrees-C in 5% CO2 in Ham's F10 medium containing foetal calf serum. Cleavage to the early blastocyst stage was obtained in vitro although most embryos displayed arrested development at the eight-cell stage. From gross morphological and ultrastructural observations a pattern of embryogenesis to the unilaminar blastocyst was constructed. Comparisons were made with the events of early development in other marsupials.
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页码:623 / 639
页数:17
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