THE HEPATITIS-B VIRUS X-GENE PRODUCT TRANS-ACTIVATES BOTH RNA POLYMERASE-II AND III-PROMOTERS

被引:144
作者
AUFIERO, B [1 ]
SCHNEIDER, RJ [1 ]
机构
[1] NYU MED CTR,KAPLAN CANC CTR,NEW YORK,NY 10016
关键词
Hepatitis B virus Xgene; pol II and III promoters; trans-activation(transcription factor TFIIIC;
D O I
10.1002/j.1460-2075.1990.tb08136.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The transcriptional regulatory activity of the human hepatitis B virus (HBV) X-gene product was investigated. We demonstrate a new property for the HBV X-gene, the strong transcriptional trans-activation of promoters for class III genes. The stimulation of RNA polymerase III (pol III) as well as pol II promoters is shown in cells transiently transfected with the X-gene, and after its stable integration into hepatocytes. We demonstrate that X-gene containing cells stimulate the frequency of pol III transcription initiation by 20- to 40-fold, and accelerate the rate of formation of stable pol III initiation complexes in a manner indistinguishable from that of adenovirus Ela protein. Since the transcription factor TFIIIC has been shown to be limiting in the formation of stable pol III initiation complexes, template commitment experiments were performed which titrate the level of this factor in extracts. We show that X-protein containing extracts are far more efficient in forming stable pol III pre-initiation complexes that cannot be competed away upon addition of a second template, indicating that TFIIIC is very probably a target of the X-protein. Thus, the HBV X-protein is apparently a member of a family of trans-activators capable of stimulating both pol II and III promoters, which includes the adenovirus Ela-protein and SV40 t antigen.
引用
收藏
页码:497 / 504
页数:8
相关论文
共 54 条
[1]   DIFFERENTIAL ACTIVATION OF RNA POLYMERASE-III-TRANSCRIBED GENES BY THE POLYOMAVIRUS ENHANCER AND THE ADENOVIRUS E1A GENE-PRODUCTS [J].
BERGER, SL ;
FOLK, WR .
NUCLEIC ACIDS RESEARCH, 1985, 13 (04) :1413-1428
[2]   SIZING AND MAPPING OF EARLY ADENOVIRUS MESSENGER-RNAS BY GEL-ELECTROPHORESIS OF S1 ENDONUCLEASE-DIGESTED HYBRIDS [J].
BERK, AJ ;
SHARP, PA .
CELL, 1977, 12 (03) :721-732
[3]   INDUCTION OF SPECIFIC TRANSCRIPTION BY RNA POLYMERASE-III IN TRANSFORMED-CELLS [J].
CAREY, MF ;
SINGH, K ;
BOTCHAN, M ;
COZZARELLI, NR .
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (09) :3068-3076
[4]  
CHISAKA O, 1987, GENE, V60, P183
[5]   A NEW DOMINANT HYBRID SELECTIVE MARKER FOR HIGHER EUKARYOTIC CELLS [J].
COLBEREGARAPIN, F ;
HORODNICEANU, F ;
KOURILSKY, P ;
GARAPIN, AC .
JOURNAL OF MOLECULAR BIOLOGY, 1981, 150 (01) :1-14
[6]   ORDERING PROMOTER BINDING OF CLASS-III TRANSCRIPTION FACTORS TFIIIC1 AND TFIIIC2 [J].
DEAN, N ;
BERK, AJ .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (08) :3017-3025
[7]   HEPATITIS-B VIRUS-DNA INTEGRATION IN A SEQUENCE HOMOLOGOUS TO V-ERB-A AND STEROID-RECEPTOR GENES IN A HEPATOCELLULAR-CARCINOMA [J].
DEJEAN, A ;
BOUGUELERET, L ;
GRZESCHIK, KH ;
TIOLLAIS, P .
NATURE, 1986, 322 (6074) :70-73
[8]   TRANSCRIPTIONAL REGULATION OF 2 SERUM-INDUCED RNAS IN MOUSE FIBROBLASTS - EQUIVALENCE OF ONE SPECIES TO B2 REPETITIVE ELEMENTS [J].
EDWARDS, DR ;
PARFETT, CLJ ;
DENHARDT, DT .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (11) :3280-3288
[9]   DETECTION OF HEPATITIS-B VIRUS-X PRODUCT USING AN OPEN READING FRAME ESCHERICHIA-COLI EXPRESSION VECTOR [J].
ELFASSI, E ;
HASELTINE, WA ;
DIENSTAG, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (07) :2219-2222
[10]  
Favaloro J, 1980, Methods Enzymol, V65, P718