NUCLEOSOME-RESTRICTED ANTIBODIES ARE DETECTED BEFORE ANTI-DSDNA AND/OR ANTIHISTONE ANTIBODIES IN SERUM OF MRL-MP LPR/LPR AND +/+-MICE, AND ARE PRESENT IN KIDNEY ELUATES OF LUPUS MICE WITH PROTEINURIA

被引：184

作者：

AMOURA, Z ^{[1
]}

CHABRE, H ^{[1
]}

KOUTOUZOV, S ^{[1
]}

LOTTON, C ^{[1
]}

CABRESPINES, A ^{[1
]}

BACH, JF ^{[1
]}

JACOB, L ^{[1
]}

机构：

[1] HOP TENON,PARIS,FRANCE

来源：

ARTHRITIS AND RHEUMATISM | 1994年 / 37卷 / 11期

关键词：

D O I：

10.1002/art.1780371118

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. To compare the humoral response to nucleosomes with the response to their individual components (double-stranded DNA [dsDNA] and histones) and to assess the involvement of antinucleosome antibodies in immune deposits in the kidney of MRL mice. Methods. We used enzyme-linked immunosorbent assays of sera and kidney eluates for antibody activity against purified nucleosomes, dsDNA, and histones. Results. Antinucleosome antibodies emerged before anti-dsDNA and antihistone antibodies. A fraction of antinucleosome antibodies reacted exclusively with nucleosomes and not with their components, dsDNA and histones. These nucleosome-restricted antibodies were detected in the proteinuric MRL mouse kidney eluate. Conclusion. Our findings support the notion that nucleosomes play a major role in the emergence of antinuclear autoantibodies and that antinucleosome antibodies might be involved in the nephritogenic process in murine lupus.

引用

页码：1684 / 1688

页数：5

共 14 条

[1] GENESIS AND EVOLUTION OF ANTICHROMATIN AUTOANTIBODIES IN MURINE LUPUS IMPLICATES T-DEPENDENT IMMUNIZATION WITH SELF ANTIGEN [J].