CHARACTERIZATION AND ASSAY CONDITIONS FOR USE OF ACHE ACTIVITY FROM SEVERAL MARINE SPECIES IN POLLUTION MONITORING

被引：180

作者：

BOCQUENE, G

GALGANI, F

TRUQUET, P

机构：

[1] Laboratoire Effets Biologiques des Nuisances, DERO-IFREMER, 44037 Nantes Cedex 01, Rue de l'Ile d'Yeu

来源：

MARINE ENVIRONMENTAL RESEARCH | 1990年 / 30卷 / 02期

关键词：

D O I：

10.1016/0141-1136(90)90012-D

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Acetylcholinesterase (AChE) activity was investigated in eight marine species of potential value for monitoring pesticides. AChE, BUChE and PChE activities were compared, and optimal conditions of extraction, storage and measurement were determined. TRIS extract buffer 0·1 M, ph8, was found to provide good specific production. AChE activity was not altered by freezer storage at -20°C. Optimal measurement temperatures ranged from 20 to 34°C, and pH was optimal between 6·5 and 8·5. To monitor the effects of anticholinesterase compounds, it is preferable to measure inhibition on plaice (Pleuronectes platessa) and common prawn (Palaemon serratus) which have high activity levels. © 1990.

引用

页码：75 / 89

页数：15

共 24 条

[1]

Bayne B. L., 1985, EFFECTS STRESS POLLU

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

COPPAGE DL, 1974, B ENVIRON CONTAM TOX, V2, P438

[4] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].